Search results for "fusion [photon photon]"

showing 10 items of 724 documents

Production, crystallization and preliminary X-ray analysis of the human integrin alpha1 I domain.

1999

Integrin α1β1 is one of the main collagen receptors in many cell types. A fast large-scale production, purification and crystallization method for the integrin α1 I domain is reported here. The α1 I domain was crystallized using the vapour-diffusion method with a reservoir solution containing a mixture of PEG 4000, sodium acetate, glycerol and Tris–HCl buffer. The crystals beong to the C2 space group, with unit-cell parameters a = 74.5, b = 81.9, c = 37.3 Å, α = γ = 90.0, β = 90.8°. The crystals diffract to 2.0 Å and a 94.2% complete data set to 2.2 Å has been collected from a single crystal with an R merge of 5.8%.

biologyProtein ConformationRecombinant Fusion ProteinsIntegrinIntegrin alpha1General MedicineCrystallography X-Raylaw.inventionCollagen receptorchemistry.chemical_compoundCrystallographychemistryStructural BiologylawAntigens CDDomain (ring theory)PEG ratioGlycerolbiology.proteinHumansCrystallizationCrystallizationSingle crystalSodium acetateActa crystallographica. Section D, Biological crystallography
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Antibacterial activity of different root canal sealers against Enterococcus faecalis

2017

Background The aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against Enterococcus faecalis, prior and subsequent to setting. Material and methods Agar diffusion test (ADT) was used for evaluating the antibacterial activity of non-set sealer while the direct contact test (DCT) was used for after setting. Results ADT: Except for TotalFill BC Sealer all the others sealers tested showed antibacterial activity. BioRoot™RCS, MTA Fillapex and Sealapex Root Canal Sealer showed the lowest antibacterial activity, a significant increase in antibacterial effect for both Pulp Canal Sealer™ and AH plus sealers were found. Significantly higher …

biologybusiness.industryChemistryRoot canal0206 medical engineeringDentistry030206 dentistry02 engineering and technologybiology.organism_classificationAntimicrobial:CIENCIAS MÉDICAS [UNESCO]020601 biomedical engineeringEnterococcus faecalis03 medical and health sciences0302 clinical medicinemedicine.anatomical_structureMTA-FillapexUNESCO::CIENCIAS MÉDICASmedicinePulp canalAgar diffusion testBacterial inhibitionAntibacterial activitybusinessGeneral Dentistry
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Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

2011

Edible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 ± 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and str…

biologyfungifood and beveragesPlant ScienceAgrobacterium tumefaciensbiology.organism_classificationmedicine.disease_causeFusion proteinIn vitroVirusMicrobiologylaw.inventionAntirrhinum majusBiochemistrylawRotavirusRecombinant DNAmedicineGenetically modified tomatoAgronomy and Crop ScienceBiotechnologyPlant Biotechnology Journal
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Sport and Quality of Life

2022

Negli ultimi trent’anni lo sport ha assunto un significato molto rilevante nella vita delle persone e, seppure con notevoli differenze, sia nei paesi a sviluppo economico avanzato, sia in quelli in via di sviluppo. A livello individuale, esso ha costituito un’area di investimento identitario quando ha assunto la forma di sport spettacolo del quale fruire, alimentando la pratica del tifo e il fandom o costituendo il modello di uno stile di vita vincente; ma anche quando ha assunto la forma di pratica del tempo libero attraverso la quale tenersi in forma, facendo crescere, in questo caso, la diffusione di una cultura della salute e del benessere. Si tratta di due modalità di approcciare al co…

but also when he took the form of free time practice through which to keep fit making grow in this case the diffusion of a culture of health and well-being. These are two ways of approaching the consumption of sports by spectators and actorIn the last thirty years sport has taken on a very significant meaning in people's lives and albeit with considerable differences both in advanced economic development countries and in developing countries. On an individual level it constituted an area of identity investment when it assumed the shape of “sport spectacle” to be enjoyed fueling the practice of cheering and fandom or constituting the model of a winning lifestyleand the two ways of use can be strongly related to the perception and assessment of the quality of life. The “semantic universes” which however connote sport and “free time sports” have often appeared polarized. Commercial sport and sports professionalism are intertwined with the institutions of economics politics and culture which above all stress its “spectacularity” in order to capture first and foremost the audience. But also the sport of leisure time is intertwined with the actions of the institutions of economics politics and culture with the difference that these stress above all its “healthy value” aimed at the “healthy and rational” investment of time in an activity which improves the quality of life in the short medium and long period. This polarization between the consumption of sport entertainment - commercial sport - and the consumption of sport as a leisure activity - sport for all - has become increasingly interconnected precisely because of the increased collective identity demand via sport. In other words we have witnessed the spread of shape of sports entertainment that recall the importance of sports for psychophysical well-being for integration and social participation for the reduction of social inequalities ethnic and cultural differences and in which the importance assumed was weakened in the show from agonism from competition from the physical confrontation between two contenders or two teams. A sport in which we act with competitors as well as against competitors. And on the other hand we have gradually witnessed the spectacularization of sports in our free time to the point that the sharing of the results obtained through the declination of a competitive spirit that presents itself as directed no longer against other contenders but against its own performance limits it has become the way in which each person makes part of his or her own life spectacular the one he often considers most authentic. The aim of this work is to describe how the intertwining between the commercial/professional dimension of the sports show and the playful/recreational dimension of sports practice are fueled by a demand and an offer of social identity that characterizes these two “semantic universes”.Settore SPS/07 - Sociologia Generale
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UJI AKTIVITAS ANTIBAKTERI DARI ESTRAK TUMBUHAN AKWAY (Drimys beccariana. Gibbs)

2010

<p><em>The plant Akway (Drimys beccariana.</em>Gibbs<em>) is endemic to Papua and is frequently used traditionally by the Arfak tribe as a medicinal plant. We extract the plant by maceration with ethanol. The antibacterial test  is done by Agar diffusion method. As microba test  we used Escheresia coli and Bacillus subtilis. Result obtained from fitochemistry skrinning of the leaf and bark contained alkaloid, flavonoid, steroid, tannin, glikosida, saponin, triterpenoid, steroid and  fenolik. Concentration of active substances from the leaf are flavonoid (0,3680%) and tanin (10.33 %); while from the bark flavonoid 18.35%  and tanin (27.65%).  Antibacterial test showed…

chemistry.chemical_classificationDrimysTraditional medicinebiologyfungiFlavonoidSaponinfood and beveragesBacillus subtilisbiology.organism_classificationchemistryvisual_artMaceration (wine)visual_art.visual_art_mediumTanninBarkAgar diffusion testJurnal Natural
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Peptide–Membrane Interactions Monitored by Fluorescence Lifetime Imaging: A Study Case of Transportan 10

2021

The interest on detailed analysis of peptide-membrane interactions is of great interest in both fundamental and applied sciences as these may relate to both functional and pathogenic events. Such interactions are highly dynamic and spatially heterogeneous, making the investigation of the associated phenomena highly complex. The specific properties of membranes and peptide structural details, together with environmental conditions, may determine different events at the membrane interface, which will drive the fate of the peptide-membrane system. Here, we use an experimental approach based on the combination of spectroscopy and fluorescence microscopy methods to characterize the interactions …

chemistry.chemical_classificationFluorescence-lifetime imaging microscopyChemistryRecombinant Fusion ProteinsSpectrum AnalysisGalaninWasp VenomsPeptideSurfaces and InterfacesCondensed Matter PhysicsFluorescenceArticleMembraneMicroscopy FluorescenceAmphiphileElectrochemistryFluorescence microscopeHigh spatial resolutionBiophysicsPeptide−Membrane Interactions FLIM Transportan 10 PhasorGeneral Materials SciencePeptidesSpectroscopySpectroscopyLangmuir
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Proteins of Human Cytomegalovirus that Elicit Humoral Immunity

1993

Several of the cytomegalovirus (CMV) genes encoding glycoproteins, structural proteins, and infected-cell proteins that elicit an immune response in human infection have been mapped. Human sera and monoclonal antibodies react with these viral polypeptides made as native molecules in CMV-infected cells, as genetically engineered proteins, as truncated derivatives expressed in eukaryotic cells, and as bacterial fusion proteins from portions of the reading frames cloned into prokaryotic expression vectors. Synthetic oligopeptides from immunodominant regions of these molecules have also been used as antibody targets. Studies on proteins encoded by reading frames UL55, UL32, and UL44, on glycopr…

chemistry.chemical_classificationHuman cytomegalovirusmedicine.drug_classvirus diseasesBiologyMonoclonal antibodymedicine.diseaseFusion proteinVirologyImmune systemchemistryAntigenHumoral immunitymedicinebiology.proteinAntibodyGlycoprotein
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Penetrant diffusion in frozen polymer matrices: A finite-size scaling study of free volume percolation

1996

The diffusion of penetrant particles in frozen polymer matrices is investigated by means of Monte Carlo simulations of the bond fluctuation model. By applying finite-size scaling to data obtained from very large systems it is demonstrated that the diffusion process takes place on a percolating free volume cluster describable by a correlated site percolation model which falls into the same universality class as random percolation. The diverging correlation length entails a pronounced dependence of the diffusion constant on the size of the simulated system. It is shown that this dependence is appreciable for a wide range of parameters around the transition. \textcopyright{} 1996 The American …

chemistry.chemical_classificationMaterials sciencePenetrant (mechanical electrical or structural)Condensed matter physicschemistryDiffusion processMonte Carlo methodThermodynamicsPolymerRenormalization groupScalingBond fluctuation modelFick's laws of diffusionPhysical Review E
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Random Forest Analysis: A New Approach for Classification of Beta Thalassemia

2020

In recent years, Thalassemia care providers started classifying patients as transfusion-dependent-Thalassemia (TDT) or non-transfusion-dependent-Thalassemia (NTDT) owing to the established role of transfusion therapy in defining the clinical complication profile, although this classification was also based on expert opinion and is limited by reliance on patients’current transfusion status. Starting from a vast set of variables indicating severity phenotype, through the use of both classification and clustering techniques we want to explore the presence of two (TDT vs NTDT) or more clusters, in order to approaching to a new definition for the classification of Beta-Thalassemia in Thalassemia…

congenital hereditary and neonatal diseases and abnormalitiesPediatricsmedicine.medical_specialtybusiness.industryThalassemiaBeta thalassemiamedicine.diseaseRandom foresthemic and lymphatic diseasesExpert opinionTransfusion statusmedicineTransfusion therapybusinessSSRN Electronic Journal
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Protective coloration of European vipers throughout the predation sequence

2020

Antipredator adaptations in the form of animal coloration are common and often multifunctional. European vipers (genus Vipera) have a characteristic dorsal zigzag pattern, which has been shown to serve as a warning signal to potential predators. At the same time, it has been suggested to decrease detection risk, and to cause a motion dazzle or flicker-fusion effect during movement. We tested these hypotheses by asking whether (1) the zigzag pattern decreases detection risk and (2) the detection is dependent on the base coloration (grey or brown) or the snake's posture (coiled, basking form or S-shaped, active form). Additionally, (3) we measured the fleeing speed of adders, Vipera berus, an…

conspicuousness0106 biological sciencesvisiongenetic structuresVipera berusAPOSEMATISMdetectionAVOIDANCEZoologyAposematismFlicker fusion threshold010603 evolutionary biology01 natural sciencesPredationzigzag pattern0501 psychology and cognitive sciencesSTRATEGY050102 behavioral science & comparative psychologyGenus ViperaSEXUAL DICHROMATISMDISTASTEFUL PREYEcology Evolution Behavior and SystematicsSNAKESanimal colorationbiology05 social sciencesflicker-fusionAnimal colorationbiology.organism_classificationcrypsisdazzle colorationZigzagDISTANCE1181 Ecology evolutionary biologyCrypsiswarning signalAnimal Science and ZoologypredationAnimal Behaviour
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